How can MMLV enzyme add cytosines to the 3' of cDNA
Hi there, I am working on the mechanism of SMART , a kind of reverse transcription strategy.
However, I am confused about the terminal transferase activity of MMLV enzyme. It have been reported since 1998, but I cannot find any resource to figure out HOW can it add Cs on the 3' of cDNA. Can anyone provide any message? I will be very appreciated. Cheers.
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Sorry, I am not familiar with the rule of this site. I apologize. And thanks for your advice.