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How to generate a mutated DNA sequence?

Hi, I'm a newbie in bioinformatics, I just want to know how to build an accurate (not random) mutated sequence of a given gene. With the mutations coming from .mafs provided by TCGA.(Just taking into account point mutations). Thanks in advance

I've been reading a lot but the more I read the less I know, so all help is welcome thanks I advance

sequence mutations
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updated by Biostar Biostar • written by mariamcurbelo2061 • mariamcurbelo2061

Give mutate.sh from BBMap suite a try. Run the command without any options to look at the in-line help.

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written by GenoMax GenoMax

You can just use the ENSEMBL server to findout variant form of gene using BLASTN usign fasta sequence or VEP-ENSEMBL analysis using VCF file. The server will give you the result of mutated sequences derived from EMS induced mutations or even from natural variations. You would get SIFT score if you use VCF file as an input to get output from each positions of the sequence corresponding to genomic data base sequence. This may be useful: A: Allele frequency visualization

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written by pltbiotech_tkarthi pltbiotech_tkarthi

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