Can I obtain my transcriptome size based on number of M uniquely mapped reads?
Hello,
I mapped my .fastq files against genome and I got the mapping quality reports. I have that 60M - 70M reads were uniquely mapped and that my reads were 100bp in size.
Can I say that 60M * 100bp (6 000 000 000 bp = 6000 Mb) is the size of the transcriptome in that sample or am I completely wrong?
Thank you.
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