hi, this is a new area far from my expertise and I want to check if a specific gene has SNPs but I don/t have MAF or any information to set the cohort number so I am not sure where to start? how to calculate?
You can try BLASTN at ENSEMBL to observe/retrieve related sequences and look for variants perhaps you could observe natural variants or EMS induced variants from ENSEMBL server. If you have VCF file, you can predict variants with SIFT score. Try this: A: Allele frequency visualization