I like to convert the BAM file to BIGWIG format I used bedtools genomeCoverage
to change BAM to BEDGRAPH but I do not know difference between below comands:
bedtools genomecov -bg -ibam
and bedtools genomecov -bg -i -g hg19.genome
because when I use the second one I faced with this ERROR:
Unexpected file format. Please use tab-delimited BED, GFF, or VCF. Perhaps you have non-integer starts or ends at line 808727866?
but the first one works well.
Also, I used deeptools bamCoverage
to change BAM to BEDGRAPH but there is no data for its output when I opened it in the UCSC.