Hello,

I performed an amplicon targeted sequencing approach, where in I designed a primer like so . I had 60 samples in my library pool. When I did MiSeq run, I see that around 80,000 UMIs (79455.41 ± 8289) UMIs for every sample had only a single read. Around 30,000 UMIs (45474.85 ± 4508) had two reads and so forth . I was wondering if any of you have an explanation for this trend for finding only a X number of reads, X times ?

Thank you. Santosh