Illumina NextSeq shorter fragment size than expected
Hi,
We were preparing library for NextSeq whole exome sequencing. Our starting concentration before library preparation for three samples were 27ng/µL, 37ng/µL and 42ng/µL. We pooled library and when we checked our fragment size before running on sequencer by Bioanalyzer, we saw fragment size of 239 base pair which is smaller than expected. I want to know on which step we are making mistake which resulted in smaller insert size than expected?
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Discrepancy Between Fragment Size Distribution and MACS3-Predicted Fragment Length in ChIP-seq Data…
written by christoph.neu •
-
Significant variation in FastQ File Sizes - WES
written by irate.pirate •
-
Tutorial: Premade library preparation – considerations, tips and tricks (II)
written by Novogene •
-
Does sequencing depth influencing ATAC peak calling?
written by Char •
-
10x 3' library creates R1 and R2 fastq files with the same read length
written by tomas4482 •
-
estimating read length and SD for Kallisto (single-end QuantSeq reads)
written by cats_dogs •
-
Weird insert size distribution
written by Zac Shi •
-
single cell RNA-seq detected genes
written by mefthymios •
-
Problems with proper interpretation of visualized bam file from Illumina Miniseq platform
written by dominik.saburo •
-
Band in Negative Control PCR, but no band in one sample with template added? Primer dimers?
written by Nicolai Skovbjerg Arildsen •
Hello BAGeno!
We believe that this post does not fit the main topic of this site.
Not primarily bioinformatics, try at SeqAnswers
For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.
If you disagree please tell us why in a reply below, we'll be happy to talk about it.
Cheers!