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How to merge read count of multiple sample in one fiel to be used for DESeq2 fold change study?

Hi, I have read count of circRNA junctions obtained by analyzing RNA-Seq data. How can I merge these files to calculate fold change using DeSeq2?Some sample may contain junctions that are not detected in other sample or replicate analysis and I need to put zero in that condition. Any help will be appreciated. .

alignment sequencing next-gen rna-seq
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updated by gayatri281 • gayatri281 • written by Neu • Neu

DESeq2 has several features for importing data: did you give a look to them? You can find them here

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written by Fabio Marroni Fabio Marroni

Have you tried full_join() from dplyr?

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written by Devon Ryan Devon Ryan

1 answer

Hi Neu, Did you figure this out? I am looking for a solution to the same problem!! Thank you!

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written by gayatri281 • gayatri281

Hi, I used ablebits-ultimate-suite-excel add-in to do this. Before doing this, I made a single table for the identical columns by merging all the sample result files and then sorted them.

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written by Neu • Neu

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