TCGA data base normalization
Hello, I am very new to bioinformatics but recently i started working on a project where i need to validates my experiments using the TCGA data base. So i have downloaded the data from the GDAC website and it's cited that the genes are normalized but apparently the normalization wass not done using the housekeeping genes. Does anyone have an idea how am i supposed to treat my genes before starting my analysis? and if i need to do a normalization with housekeeping genes which one (human samples) should i be using? Thank you in advance for your help. Best regards, Safa Azar, PhD
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Hi safa.azar, which data did you download and in which format. What is the question you want to answer and which steps did you already take. Please read Brief Reminder On How To Ask A Good Question as an inspiration on which information should be provided at minimum in a question.