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Kevin Blighe
DESeq2 comparison of normalized counts from different experiments
Hi,
I'm putting together a database to compare gene expression across many different RNA-seq experiments. I have multiple DESeq2 normalized counts matrices from the different experiments.
My question is am I able to merge these normalized counts matrices by gene names and directly compare the gene expression across all of these experiments? Or will I have to re-run DESeq2 with all samples together to have the normalization done together?
Thanks, Matt
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Matthias •
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You'll want to run them all together, otherwise the gene-dispersion estimates won't be accurate, meaning your counts won't be comparable across experiments. You'll likely still run into batch effects, but at least you can try to account for those.
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