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De novo Genome Sequence

How to do genome sequencing of an organism without knowing the genome size?

Thanks.

genome

could you explain your question little wider, in terms of what kind of organism it is, what you're going to achieve and all? One more thing what does it mean genome sequencing of an organism without knowing the genome size?.

Hello debashis.panda814!

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Not primarily bioinformatics, try at SeqAnswers

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This has been rightly closed, but to answer your question, you don't really need to know the genome size. You might want to so you can estimate coverage etc, but it would be fine to estimate off the general size of other organisms of that class - e.g. most bacteria are somewhere sub 10 Mb.

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