This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Cross-platform Assembly (PacBio & Ion Torrent)

I have sequenced a genome of a cyanobacterium with two different sequencing methods, PacBio RS and Ion torrent. I wanted to know if there is a tool to compare both sequences since I know that one has more sequence than another and I want to know where the missing gaps are.

Thanks very much

sequencing assembly genome alignment
• 1,167 views • link
written by l.algibe • l.algibe

The term you are looking for is a "hybrid assembly". Using that for googling will give you plenty of hits.

• 0 views • link
written by WouterDeCoster WouterDeCoster

Thank you very much, I will try to find it like that

• 1 views • link
written by l.algibe • l.algibe

Use the pacbio assembly only, and if you like map Ion torrent reads to the pacbio contigs. There is an absolutely massive difference in read length and quality between the two!

If unclear : ion torrent is pretty awful

• 1 views • link
written by colindaven colindaven

0 answers

No answers yet.

Log in to answer this question.

More posts like this