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Pacbio Sequencing Reads Data Output

Hi. I am very keen to know more on how different sequencing platforms generate their data and how their raw data looks like. I have come across the uniqueness of colour spacing for SOLiD ABI and flowspace for Ion Torrent. My next target is PacBio data. Does anyone know more about PacBio's sequencing reads data output? Is there anything unique about the way they generate their data? I am unable to find any solid information online. Thanks.

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updated by Biostar Biostar • written by deliciayong • deliciayong

Hi Alex. Thank you for the suggestions. Shall give it a go.

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written by deliciayong • deliciayong

1 answer

The PacBio flowcell generates bas.h5 format, here is more information. Installing the SMRT Analysis portal is the easiest way to deal with the data. Be sure to follow the installation instructions carefully, particularly the bit about installing as a new system user on Linux. The package includes the blastr program for sequence searching.

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written by Alastair Kerr Alastair Kerr

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