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How to perform mass spec analysis

Hi ,

I Have 18 iTRAQ.raw file got it from orbitary mass spec machine. I am not sure which sample is control and which sample is test. I have one gene for which i want to get protein sequences. i have made amino acid fasta file of this gene. Now i want to look for protein sequences in my mass spec sample. I tried using MAXQUANT TPP nothing is working fine . I tried using MASCOT server but once i click on start run it is not showing me any display.

Kindly help me to know how should i get this sequence

mass spec analysis

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