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How are amino acid sequences obtained from proteomics studies?

Naive question here from a genomics person. How are proteomic results (e.g. from mass spec of extracted tissues) linked to annotated protein sequences? Can proteomic studies like mass spec directly give you the amino acid sequence, which you can then reverse-translate?

For example, if someone identifies the proteins in the glands of an insect, how do they go about getting sequences for those proteins?

gene protein proteomics mass spec
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written by amango • amango

1 answer

Take a look at the first couple of videos for intro. to proteomic data analysis.
Ultimately you are doing similarity searches to identify the possible AA sequence. An example of the program used is Mascot.

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written by GenoMax GenoMax

Exactly what I need, thanks.

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written by amango • amango

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