I have the amino acid sequence of a gene of interest. I want to identify all the different homologs in different species, and assemble it in a single FASTA file format. Which I then want to input into a sequence similarity network (SSN) tool, to find how the different homologs from different species cluster.
My problem is: how do I get this list of homologs into one FASTA file? I tried two approaches. One is find the protein on Kegg database, then I click on 'Orthologs'. This gives all the homologs in the KEGG database (about 2500). But I don't see a way to download the amino acid sequences in one go, unless I click each homolog indvidually and copy the aa sequence.
The second option is to do a BLASTP usign RefSeq as the search database. This gives me only a limited number of hits (restricted to 100), and furthermore, I still can't figure out how to download the FASTA sequences of the hits in one go.
Can someone please offer some help? Thanks in advance!
3 answers
Hi Using BLASTP you can get your homologs. Hits by default are restricted to 100, you can increase this to upto 20000 using the web interface of BLASTP where u can click on advanced parameters to get the dropdown menu for adjusting the max number of hits.
On the result page, just select all and there will be options to download the complete fasta sequences as well as you can download the aligned sequences if you want.
Hope it helps.
Thanks Swati
Have you looked at NCBI's homologene database? Ensembl also has a similar solutions available. If your protein is a standard accession then you should be able to find it in these databases.
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what kind of species are we talking about here? if tit's plants (or "related") you should have a look here: PLAZA ; the whole purpose of this resource is exactly homology, gene families etc ...