Correct Interval Coverage data for overall number of raw reads
Hello,
I have two datasets with Interval Coverage metrics that I would like to compare. However, both datasets have a significant different amount of raw reads. I would like to normalise or correct for this different amount of raw reads in order to make the interval data coverage comparable.
Any suggestions? I thought of correcting for an effect of raw reads or standardized all of the interval coverage data.
Any help would be greatly appreciated.
Thank you!
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Two ways of doing it:
- Downsizing: can be done using samb: http://lomereiter.github.io/sambamba/
- Normalise the data to a mean of zero and sd of 1
I guess I answered my own question... :)
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