This is probably a very basic question but would the TaqMan™ Advanced miRNA Human A and B Cards a single or dual color microarray. I am observing a batch effect with my samples being analyzed at different times. I have used batch in the linear model and also correct for batch bias with combat and sva before running the linear model. While they seem to do the job I am worried they are 'over-correcting' and i am losing true biological results. So I wanted to look at limma package as there are some functions within this package to "correct" for this however I am unsure which method from this package is appropriate based on this type of array.
Any suggestions would be greatly appreciated.
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Since the listing for the product shows only one dye (
FAM) this should be a single color detection.