Biostar Beta. Not for public use.
Question: Align two single cell experiments
Entering edit mode

Hi guys, I'm trying to align two single cell experiments using the Seurat package. According to the tutorial here: the standard pipeline first performs the FindVariableGenes step and then creates an object from the union of the two datasets. I would like to perform the regression on mt-genes and on cell cycle genes. My question is: when to perform the regression? On the single objects independently before the union or on the object from the union?

Thank you in advance!

ADD COMMENTlink 15 months ago elb • 160 • updated 15 months ago Michael Dondrup 46k

Login before adding your answer.

Similar Posts
Loading Similar Posts
Powered by the version 2.0