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CAGEFightR to get CTSS from CAGE error
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14 months ago
bharata1803 • 420


So, I got bam files from CAGEseq experiment from FANTOM5. I realigned it to Ensembl GRCh 38 90 using bwa and then extract the bigwig file using deeptools bamCoverage.

Then I am trying to get the CTSS using CAGEFightR from the bigwig files (plus and minus strand) I have generated.

Using the quantifyCTSSs from CAGEFightR function, I got error "Error in asMethod(object): all the ranges in the object to coerce to GPos must have a width of 1"

What is wrong with my method?


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