I'm new to the field of metagenomics and I have been reading research work done on binning in metagenomics. I came across two types of metagenomics binning tools;
- binning of reads (before assembly) - ex. MetaProb, BiMeta
- binning of contigs (after assembly) - ex. MaxBin, MetaWatt
My understanding is that we bin reads and contigs to identify which group of closely related organisms or OTU they belong to as there can be a huge number of organisms in a metagenomic sample.
Can someone please explain me why do we do binning before (reads) and after (contigs) assembly, what is intended to be achieved and the consequences of each?
Sorry if this is a naive question. I would really appreciate an explanation. :) Thank you!