Bowtie indexes for custom reference genome
Hi all,
I want to make my own bowtie indexes to align the sequences to a custom reference genome.
Is it necessary only to create a fasta file and then bowtie-build to have proper indexes for mapping or I should also create a GTF file? If so, how do you create a GTF file?
Thank you for youe help
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f.rivello •
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Bowtie can index from a set of DNA sequences. It produces six file which constitute the index. For more details please check this link manual section on index building
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Thank you. A GTF file not necessary to buid a custom bowtie index, but only a fasta file with my sequences right?
Sorry for the question, I am a beginner in the field.
yes we don't need gtf file to build genome index. Good luck
Bowtie is a DNA-seq aligner, not a splice-aware RNA-seq aligner. Therefore, it does not even has an option to accept a GTF. All you need is a fasta of the reference genome. Splice-aware aligners need information about splice and exon positions, that is why these files are necessary for e.g. tophat, hisat, star etc.