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Problem running MAFFT

I'm trying to run MAFFT for aligning sequences in .fasta format. I'm working on Ubuntu and using MAFFT version 7.407.

I got MAFFT running on a set of 100 sequences but it's giving me the following error on 500 sequences:

Allocation error ( 3448 x 3448 doublemtx )

I'm running MAFFT using the options --localpair --maxiterate 16 --phylipout --inputorder

Could anyone please help me out as I'm pretty new to this?

alignment sequence

You are probably running out of memory. See the tips page for suggestions on how to handle big datasets.

Wow, this really solved my bug: ApplicationError: Non-zero return code 1 from 'mafft Ref_andOneMore.fasta', message 'nthread = 0' , Time-expending googling didn't help, but your recommended page ("tips") helped.

So I used the --memsave option and it was enough to get it run.

mafft --auto --memsave Ref_andOneMore.fasta > out3.txt

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