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Question: GRO-seq - low Same / Diff strand fold enrichment
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By using makeTagDirectory (HOMER) to analyse GRO-seq data, I am getting low value of Same / Diff strand fold enrichment (below 6)

Perhaps, because of this issue, HOMER is returning "Guessing sample is ChIP-Seq" rather than RNA-seq. According to the manual (

Same/Diff fold enrichment should be high


If HOMER doesn't guess the sample is strand specific RNA-Seq, there may be problem with how the data was mapped or with how the file is formatted.

I have this issue for 2 samples out of 10. All the files were processed equally and mapped to the human genome-hg19 using Bowtie2 followed by samtools view/sort.

Any comments why I would expect high values for Same/Diff using GRO-seq data?


ADD COMMENTlink 16 months ago l.moreno.uva • 0 • updated 14 months ago Biostar 20

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