Thanks for the reply ATpoint, Here is my Adapter removal command:
cutadapt3 -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT -o Sample_1.fastq -p Sample_2.fastq Sample1_1fastq Sample1_2.fastq
I am not getting any warning, while aligning with raw unfilterd data, with filtered data only am getting warning.