I am getting MISO bayes factor 1000000.0 , Is it correct value and what does it mean?

Hi all,

I am running miso pipeline for mRNA seq data, I have successfully installed and ran the pipeline. But I have some problem with results interpretation. For some alternative splicing events, I am getting high values for Bayes factor like this 1000000.0.

Is this really correct, If correct what does it mean? If not what may be the issue?

Thanks

miso rna-seq alternative splicing

• 1,450 views • link

written 8.0 years ago by Sarath Kumar •

0 answers

No answers yet.

More posts like this

Any tool to detect differential splicing of a gene between samples?
written 4.5 years ago by Apex92 •

Hi all - is there any well know software or a website that can check for significant alternative events (different splicing junctions) of a gene? …
alternative splicing: very different outputs from rMATS vs altanalyze
written 4.8 years ago by smurph50 •

We are comparing two RNA-seq datasets (read length = 150nt, depth = 50M reads, 2 conditions, n = 4 per condition) for alternative splicing. I …
Alternative splicing convention
written 7.3 years ago by ognjen011

I am reading the [`SUPPA2` github page][1] and can't truly understand the symbolic conventions for the events. What is a dash and what is a …
junction_annotation.py: How many 'novel' splice junctions/splice events are resonably expected from…
written 7.5 years ago by RNAseqer

Hello all, I was just wondering what a reasonable percentage of 'novel' splice junctions/splice events is for human RNAseq data using the program junction_annotation.py. I …
Is it possible to analyze differentially expressed genes without sample replicates using DESeq2?
written 7.9 years ago by Sarath Kumar •

Hi everyone, I am trying to run mRNA seq pipeline (HISAT > FeatureCount > DESeq2). I have sample data without replicates (1 normal and 1 …
differential survival genes and survexpress
written 8.1 years ago by Omics data mining

Dear all I have approx 200 genes which are getting differential expressed among short and long survival in cancer. For further interpretation, I wanted to …
Annotations for use with MISO and sashimi_plot
written 9.0 years ago by mail2steff

Hello, I am using MISO for studying alternative splicing sites in *Arabidopsis thaliana*. MISO needs different types of annotation files namely: Exon-centric annotations, Isoform-centric annotations …
How to get Cufflinks data for MISO alternative splicing events?
written 10.6 years ago by thefirstrealace •

Hello, I am currently doing an analysis on splice junctions at alternative exons, that belong to exon-skipping (ES) events. Therefore I have done the following …
RNAseq project ideas for young intern with MISO?
written 11.0 years ago by cindyy101 •

<p>Hi I'm a young high school intern doing working with RNAseq this summer. These few weeks I've been looking at a specific gene in neuronal, …
In search of "good" alternative splicing datasets for benchmarking purposes...
written 11.5 years ago by Bohdan Khomtchouk

I'm looking to benchmark some known splicing algorithms against each other (e.g., MISO, MATS) to examine how they handle datasets relative to one another with …