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Split-Seq full length

Hi,

Does the Split-Seq protocol yield full length or 3' sequencing?

From the paper it looks like it is 3' but they also use random hexamer priming.

Thank you!

rna-seq
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updated by h.mon h.mon • written by Sarah • Sarah

I'll happily make way for people if they have answers but I suspect this question might be better over on SeqAnswers instead.

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written by Joe Joe

1 answer

According to the review A practical guide to single-cell RNA-sequencing for biomedical research and clinical applications, it is 3'-end sequencing.

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written by h.mon h.mon

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