Hi,
I am looking for possible enhancer sequence and am looking at available Chip Seq data done by other researchers. The available data is posted on Facebase website, which is a website for craniofacial researchers (https://www.facebase.org/data/record/#1/isa:dataset/accession=FB00000860). This data is visualized in many BIGWIG tracks on to a genome browser with just chromosome positions and many bar graphs. I have never done Chip Seq myself and do not understand what these tracks mean. Also, can someone explain why the peaks appear at different positions in different tracks? Thank you.
1 answer
The link is broken, but it doesn't matter for your question.
Any genome browser primarily displays the signal of a ChIP-Seq/RNA-Seq experiment ( and also all other -Seq experiments, variants, peaks, repeat-elements, etc etc etc etc).
For ChIP-Seq, what you see by a bar graph is the signal of a particular protein/histone activity at a given genomic location. If you are looking at a bargraph of a FOXA2 ChIP experiment, that means whenever there is a binding of FOXA2 at a genomic location, there will be bars (signals) and the height of bars represents the strength of FOXA2 protein (TF) binding. You need to understand what is the output of a ChIP-Seq experiment and what does it mean by a transcription-factor ChIP-Seq and Histone modification ChIP-Seq, which are two major readouts commonly used to define enhancers/promoters and to gain insights into genome cis-regulation.
So each track measures a different transcription factor or different histone modification signal, hence you see bars appearing at different places. Combinations of different assays are used to define enhancers/promoters etc
A: How to find out whether Chip-seq peaks falls within promoter or enhancer region
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1) Link was broken for me
2) The peaks must correspond to a protein binding the DNA at those spots, as detected by a given antibody. Maybe the peaks are at different spots due to different antibodies or different conditions.
Need a picture or working link to help any more.