Cogena framework for RNA-seq / minimum sample size

Question

[IrK]

Dear Zhilong Jia,

I am wondering if Cogena framework is a statistically appropriate method for co-expressed gene set enrichment analysis of RNA-seq dataset, considering that input dataset (gene counts) is appropriately normalized? What is the minimum samples number that can be used? Would 3KOs vs 3WTs be sufficient?

Thank you.

[Kevin Blighe]

Calling Zhilong Jia