stringtie and fpkm and coverage

Hello all, I have assembled transcripts from a tissue using StringTie and I want to filter transcripts so that I can get rid of false positive results. What value should I consider fpkm or coverage and what should be minimum threshold?

rna-seq assembly

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written 8.4 years ago by qudrat

You could adapt the ExN50 from Trinity.

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written 8.4 years ago by h.mon

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