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Makeblastdb Issue Buffer Overflow

I am running into a makeblastdb issue. The error I am receiving is: * buffer overflow detected *: makeblastdb terminated ======= Backtrace: =========

I do not know what the issue is. I have run this program with other fasta files and had no issues with it. Here is the command I am running along with the header of my fasta file:

makeblastdb -in Paraeurythoe_californica_with_annotations.fasta -dbtype prot -parse_seqids -out test1

>Pareurythoe_californica_comp62_c0_seq1_BlastHit_gnl_BL_ORD_ID_305067_gi_82185333_sp_Q6NS09.1_TM45B_XENLA_RecName:_Full=Transmembrane_protein_45B_6.37168e_07_comp62_c0_seq1_len=233_path=[1:0_232]
ALPISCLYCSWWLH*RSLRRVGRI*TSRFTREYSEFSTLHHVSFLWYCGCD*NFASETVSRAKRTGIRCYGYGICNY

Any help would be appreciated

  • Gio
blast database fasta
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updated by GenoMax GenoMax • written by Gio12 Gio12

1 answer

Your fasta header is too long and especially the gene-name/ID "field" (=everything up to the first space)

Shortening that should resolve this.

• 1 views • link
written by lieven.sterck lieven.sterck

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