Hi I am studying polyadenylation of ribosomal RNA in different yeast mutants. I am interested in knowing how does the coverage of ribosomal RNA locus in my oligo-dT purified samples changes in various mutants. After alignment by HISAT2, I converted bam file to bigwig file by deeptools with a default setting and viewed in IGV . I also viewed bam file directly in IGV they looks different. From bigwig file it seems to I have lots of read in rRNA locus. I will be really happy to know whey they took different? Looking forward to hearing from you.