identifying contribution of specific cell subpopulation from mixture,with RNA-Seq data
If the mRNA- Seq data is acuquired from a mixture of cells, such as whole blood cells, can I use some bioinformatics methods to distinguish the contribution of each cell subpopulation? Are there some databases provide with reference cell population expression profile, and corresponding packages?
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Qingyang Xiao
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Not unless the RNA was tagged at the cellular level during library prep (e.g. drop-seq, 10x single cell etc.)