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Question: Using R bioconductor for differential gene expression
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I just want to know how to use R bioconductor for the 3 sets of microarray data of schizophrenia and whats it's steps, please answer me?

Thank you.

ADD COMMENTlink 2.1 years ago shuklapriyanka287 • 0 • updated 23 months ago zx8754 7.5k
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If you google the words in the title of your post, you'll find an array of good documentation. Please make some attempt to do some research before asking a question. The result will be better for everyone.

Read the Limma Userguide, as mentioned by Fereshteh, it's like a mini-bible on differential gene expression measurements.

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seidel
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Hello shuklapriyanka287!

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Too broad.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

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ADD REPLYlink 23 months ago
zx8754
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First: normalize your data based on the array (CEL or txt)

sharing some naive codes for microarray normalization in R with whom are too new in R alike me

Second: use limma for differential expression

https://www.bioconductor.org/help/course-materials/2005/BioC2005/labs/lab01/estrogen/

When I did not know how to use R, I used to use geworkbench to do so

http://wiki.c2b2.columbia.edu/workbench/index.php/Download_and_Installation

Just download and install that, then upload your data and use t-test or so on for differential expression

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