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STAR analysis and alignment

Hello everyone, Can I run STAR alignment tool on human genome using 16 GB RAM Linux system?

rna-seq alignment

3 answers

try HISAT2 - they state that you can align a human genome on a desktop computer in their protocol. Note, however, that the index building will require more RAM but you can download standard indexes from their homepage.

there is option --sparse which reduces the RAM needs to 16 GB for human genome

Actually typing : "star alignment requirements" on google give you what you need Then just Ctrl+F on publications using "require" key word

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3530905/

STAR’s high mapping speed is traded off against RAM usage: STAR requires ∼27 GB of RAM for aligning to the human genome

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4631051/

RAM requirements: at least 10 x GenomeSize bytes. For instance, human genome of ~3 GigaBases will require ~30 GigaBytes of RAM. 32GB is recommended for human genome alignments.

That will be hard with 16GB but give it a try imo !

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