detecting antisense transcripts

Question

[vm.higareda]

Hello

I am analyzing a single end strand specific (dUTP), transcriptome of D.melanogaster. I have already mapped and counted my sequences using:

hisat2 --rna-strandness R htseq-count -f bam -s reverse

Now, I would like to detect antisense transcripts How could do that?

Thanks

[h.mon]

SeqMonk has an antisense transcription pipeline you could try.

If you want to dissect the problem in more detail, you can try the approach from Antisense transcription - how to detect it?.