detecting antisense transcripts
Hello
I am analyzing a single end strand specific (dUTP), transcriptome of D.melanogaster. I have already mapped and counted my sequences using:
hisat2 --rna-strandness R htseq-count -f bam -s reverse
Now, I would like to detect antisense transcripts How could do that?
Thanks
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vm.higareda •
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SeqMonk has an antisense transcription pipeline you could try.
If you want to dissect the problem in more detail, you can try the approach from Antisense transcription - how to detect it?.