Sringtie gff output has "STRG." instead of genid
0
1
Entering edit mode
6.3 years ago
mkh ▴ 60

Hi all

I am trying to do genome assembly by stringtie software. The command that I am using is as follow:

  • " stringtie -p 2 -G dmel.gtf -o out.gtf STAR.bam "

  • The input bam file is from STAR.

The output file is look like this:

3R      StringTie       transcript      1       656     1000    -       .       gene_id "STRG.1"; transcript_id "STRG.1.1"; cov "3.112805"; FPKM "382.908844"; TPM "572.137207";

3R      StringTie       exon    1       656     1000    -       .       gene_id "STRG.1"; transcript_id "STRG.1.1"; exon_number "1"; cov "3.112805";

3R      StringTie       transcript      734     1776    1000    -       .       gene_id "STRG.2"; transcript_id "STRG.2.1"; cov "6.242569"; FPKM "767.903931"; TPM "1147.391479";

3R      StringTie       exon    734     1776    1000    -       .       gene_id "STRG.2"; transcript_id "STRG.2.1"; exon_number "1"; cov "6.242569";

So as it shown above there is no "transcript_id" and "gene_id" corresponding to those in the input gtf file. Stringtie end with no Error so i can not understand what is wrong with my inputs.

Thanks

Assembly RNA-Seq • 3.8k views
ADD COMMENT
0
Entering edit mode
ADD REPLY

Login before adding your answer.

Traffic: 1276 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6