Possible to directly compare RNA-Seq and DGE data?
Hello,
I have sequencing data from RNA-Seq and DGE (Digital Gene Expression) for the following conditions:
RNA-Seq: Condition A + Condition B
DGE: Condition B + Condition C
Would it be possible to compare Condition A against Condition C from different methods? These experiments were done at different times so we no longer have the samples available to run them on the same platform.
Thanks!
1 answer
Unfortunately, probably not.
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Sean Davis
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To add to Sean Davis : No, library prep/quantification methods will seriously bias the result. You would be doing a technical comparison of the method RNA-seq with the method DGE, and not the underlying biological comparison of condition A vs condition C.