What is the orientation of the sequences in illumina paired end output file?
In a illumina paired end sequencing output file, are both the two corresponding sequences in the same orientation (like both are in 5' to 3') or are they in different orientaions (like one in 5' to 3' and other one in 3' to 5')?
• 4,959 views
•
link
updated
by
Fisrt_sight407 •
•
written
by
Shanika •
•
written
by
Shanika •
1 answer
This post may show you what you want here
• 0 views
•
link
written
by
Fisrt_sight407 •
Log in to answer this question.
More posts like this
-
Bowtie2 and BWA-MEM not working
written by Matt01 •
-
Why can’t we have full-length transcripts with droplet-based technologies ?
written by sagane.joye •
-
Watson and Crick strand in Illumina sequence by synthesis
written by hakuna081017 •
-
Advice for trimming asymmetric adapters for paired end sequencing library
written by song.michael12 •
-
Please advise me on PE sequencing reads prepared by NEBNex kit and Hiseq2000
written by seta
-
Custom sequences vs supplied sequences in trimmomatric
written by ofonov •
-
Aligning two genomes in fastq.gz
written by sukesh1411 •
-
merge .fastq reads of different lengths and align
written by User6891
-
Orientation in paired-end sequencing?
written by John Smith
-
Illumina Genome Analyzer - Single End Vs Paired End
written by Bio_X2Y
Sequence reads are always in 5'-->3' orientation. See: Orientation in paired-end sequencing? . Mappers will automatically reverse complement reads (as needed) when mapping the reads.