So there's an overlap of 50 bp between the mates.
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deepti1rao •
Insert size provided by bbmerge
My illumina paired end reads are 150 bases long.
I ran bbmerge to check the insert size. Following is the link for the screen shot.
How can I have an avg insert size of 243 and a maximum of 291 when my reads themselves are 300 (2*150)? Does insert size here mean the inner distance (distance between R1 and R2)? I'm aware that there's ambiguity in the definition of insert size.
So, shall I consider the avg insert size to be 243 + 150 + 150 = 540 ??
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GenoMax
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deepti1rao •
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1 answer
See the explanation in this thread: BBMap and BBmerge insert sizs
|------------------------------>100 bp| R1 - 150 bp
|-------------------------------------| Fragment 250 bp
|100 bp<------------------------------| R2 - 150 bp
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The insert-size in paired-end data
I went through this Sej.I want to know how I can have such small insert sizes if I have reads which are 150 each.
Insert sizes are a characteristic of your libraries and have nothing to do with read lengths.