More posts like this
-
Stringtie does not work with NCBI GTF file?
written by 100dustedclays •Hi all, I wanted to rerun my DGE analysis to see if there were any differences between HTseq-count -> edgeR and StringTie-Ballgown. However, when I …
-
Including unannotated transcripts in ballgown diffexpression analysis
written by gabrielpenun •I am interested in some potential novel transcripts in an RNA-Seq data set, so I am producing a transcriptome with stringtie that does not rely …
-
Stringtie output shows no expression for reference genes
written by samhairle •Hi all, I am using the stringtie - ballgown pipeline from https://ccb.jhu.edu/software/stringtie/index.shtml?t=manual#de and I am running into an issue with the outputs. My resulting ballgown …
-
StringTie error input file not sorted, even though it is
written by sg197 •I've been following [the stringtie pipeline for differential expression](http://ccb.jhu.edu/software/stringtie/index.shtml?t=manual). I'm up to the stage of `stringtie -e -B` where I'm using the merged transcript file …
-
How to create table counts for Ballgown after performing StringTie for Estimateion transcript abund…
written by hirahameed222 •I am using HISAT2 ,Stringtie & Ballgown for differential expression analysis of wheat RNA-seq data. According to this Protocol in a Step **Estimate transcript abundances …
-
Generating FPKM matrix accross all samples after stringtie
written by ever_wudi •Hi, I finished the "stringtie --merge" step and used "stringtie -e -B -G" to reestimate fpkm for each sample and generated the ballgown folder. Now …
-
Stringtie error message
written by saj98Hello everyone I am using the following command which already explained in the nature protocol paper stringtie –e –B -p 8 -G stringtie_merged.gtf -o ballgown/WS021/WS021.gtf …
-
StringTie -e estimate parameter
written by k.mooo •I am performing RNA-seq analysis using the following pipe - HISAT2 - StringTie - Ballgown I used a cell-type specific enrichment method to target reads. …
-
Error message whengenerating read coverage tables for Ballgown by stringtie
written by hu.guangzhen9 •Background Differential expression analysis workflow by stringtie includes the following steps: 1. for each RNA-Seq sample, map the reads to the genome with HISAT2 using …
-
Can't group samples in StringTie for differential expression
written by CandiceChuDVMHi all, I have performed StringTie for individual samples and --merge for all samples by implementing the following commands: $ stringtie $PATH1/sample1.aligns.sorted.bam -G $ANNOT -p …
-B option is to output for downstream ballgown analysis. Look at your output. If the output from StringTie resulted in .ctab files, then, i guess, you are good to go.
Yes, I have the ctab files as the output. So does the option -e mean only for transcript annotated in the reference genome?