Stringtie error message
Hello everyone
I am using the following command which already explained in the nature protocol paper
stringtie –e –B -p 8 -G stringtie_merged.gtf -o ballgown/WS021/WS021.gtf Bam/WS021.bam
The issue it gave me the following error
Error: input file –e cannot be found!
However, when I delete -e and -B options, it run fine. Can anyone explain for me the problem? I appreciate your time and help
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This looks like a positional parameters/named parameters error. Maybe the program is not equipped to handle
-ein the position you're passing it in?Try putting the -e and -B at the end of the command, maybe.
Has the above command line been copy / pasted from your scripts? Note that
–eand–B(with dash) are different from-eand-B(with hyphen), and command line parameters are indicated by hyphens, not dashes.