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Duplication level (RNA)

Hello there, I'm trying to figure out how to evaluate this graph. ![enter image description here][1]

Since I am a newbie I would like to understand a bit more the meaning of the two lines and their trends. Basically, I supposed that about 50 percent of the sequences are not duplicated with another peak at more than 10. but why there are 2 lines?

thanks

rna-seq

1 answer

Take a look at this blog post by Dr. Simon Andrews, author of FastQC.

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