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If I have a miRNA purified sample, can I use TruSeq Stranded mRNA LT for library prep?

Hello all,

I've been looking for papers but I am still in doubt If could use the kit that I have in the lab, TruSeq® Stranded mRNA LT (Illumina), for library prep of my miRNA samples. I already did the RNA extraction, using miRNeasy Mini Kit (Qiagen), and enrichment of miRNA and RNAs <200 nucleotides, using miRNeasy Mini Kit (Qiagen). Does anyone that knows better about this, could please tell me?

Thanks

rna-seq

You should ask this question over at SeqAnswers. This post will likely be closed since this is not a bioinformatics question.

I am sorry. I thought I'd seen these kind of questions here. My bad!

Hello valopes!

We believe that this post does not fit the main topic of this site.

Not a bioinformatics question.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

Yes, I do agree! I am sorry It was my mistake. Thanks for letting me know.

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