How to find MW of protein by the results got from MS and SDS-PAGE.
I have done mass spectrometry and sds-page experiments to find expressed proteins in gallus gallus specie. I got results from string database, these are the files which i have right now string data base results. The results got from sds-page are here sds page result.
As i already mentioned about mass spectrometry and string database results, so i have the protein sequences.
My problem is that, how can i know the names of that expressed proteins which expressed in sds page .
as i mentioned above.
Please help me, your answers will be appreciated.
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Jean-Karim Heriche
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arjahejo •
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arjahejo •
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MASCOT searches are generally used for MS results. Have you trried those?
Please don't use
tooltag for this question.Tooltag is used for posts containing announcements for new tools.Don't post the same question multiple times. Explain why the previous answers are not suitable. Also don't copy links as images, this is useless.
For people interested, the other posts are here and here.
It seems you're dealing with data in a paper, not your own data. Can you try to make your question clearer ? Are you asking us if it is possible to reassign an identified protein to a band in a gel in a published paper ? If so, unless the paper explicitly mentions which proteins came from which band, you can only guess.
Respected Jean, i am highly thankful of your interest which show me on my posts, but unfortunately i am not cleared yet, thats why posting it with new ways to make my question clear for you people. My limit for posting here is limited i can not post more, firstly could you please send me your email as i can explain what ever it is.
Now the answer of your question is that, its data given by my professor, who said to find the names of protein, i am writting paper from that data, these table which i post here is results of my paper which is not publish yet. I moved on discussion in the paper now i dont know the names of those protein which expressed in sds-page, i have only molecular weight. I spend more than 3 months on writting this paper, now i am overwhelmed taking to much time and output is still beyond my understanding.
What you don't seem to understand, which we have already said to you, is that you cannot get protein names from your SDS-PAGE. You might as well forget about it that experiment. You have to use your mass spectrometry data. try some of the links, like MASCOT, that Jean provided. If that doesn't work, try searching the internet for papers and protocols for turning the type of protein data you have in to something useful (you haven't told us whether you have just the actual mass spectrum itself, or peptide fragment sequences etc.)
You can probably go and speak to the technicians that run your Mass Spectrometry facility.
When I have done proteomics in the past, I have used Scaffold, but the data that comes from the machine requires converting before you can use it (our technicians perform this step, so I can't help you there I'm afraid).
Hello arjahejo!
Duplicate posting
For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.
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Sorry to posting the same question again, actually i am not cleared yet, and trying to ask the same thing in different ways. This website is the only hope for me. I want to complete this given task within given time please support me. I am worried about all things which i mentioned in my posts, and i am still not cleared and my posting is limited over here.
Kindly help me