Thanks Sir, It is working.
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How to Load raw data in Trimmomatic in Galaxy? It does not assess to load in reads in FASTQ format, in Galaxy version 0.4.3.1
fastqsanger or fastqsanger.gz. If they're already actually of that type and just labeled fastq (all sequencing data produced in the past ~5 years is fastqsanger) then change the datatype. If not, use fastqgroomer to convert it to fastqsanger.Log in to answer this question.