fuzznuc helped me to solve the problem. Thank you very much!
Hi guys. I'm beginner of bioinformatics.
In order to evaluate binding property of a compound which can selectively bind short DNA sequence in genome, I want to locate short sequence, for example "5'-GAATT-3'", from fasta file (genome) and make a heat map counting the number of the short sequence in windows. How should I do?
I'm not good at ruby or perl, and I'm glad if you give me solution in python, R or Linux command line.
1 answer
This might be what you want: http://emboss.sourceforge.net/apps/cvs/emboss/apps/fuzznuc.html
However, spend some time reading this: How Do I Search A Genome For A Known Motif, And Get An Interval File Of All Instances Of The Motif?
So you should to post the answer for the other !
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Hi ,
If i resume you want to : -step 1 : "grep" all GAATT you have in you genome fasta -step 2 : note the coordinate of motif matching ? -step 3 : make a plot of you motif along your genome fasta ?