Hadn't seen this, very helpful - thanks.
Chromatin Accessibility Data and RNA-seq
Hi all,
Wondering about opinions of interesting ways to intersect these two data types? Such as DNase or ATAC-seq and RNA-seq of the same cell type, same conditions etc. Only a few papers thus far trying to correlate the two data streams, wondering about opinions out there. Thanks.
Rob.
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A paper just came out that addresses that question: Modeling gene regulation from paired expression and chromatin accessibility data.
Hopefully that is not what inspired your question.
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Have you succeeded in running this analysis? I'm just attempting to run it with the demo dataset, but I have a problem at step 3, where I get the following:
step 3: calculate opn... mkdir: Enrichment: File exists
Position file = region.bed
Genome = mm9
Output Directory = ./.
Using actual sizes of regions (-size given)
Fragment size set to given
Will use repeat masked sequences
Will not run homer for de novo motifs
Will search for known motifs in file: ../../../Data/all_motif_rmdup
Found mset for "mouse", will check against vertebrates motifs
Peak/BED file conversion summary:
BED/Header formatted lines: 0
peakfile formatted lines: 0
Peak File Statistics:
Total Peaks: 0
Redundant Peak IDs: 0
Peaks lacking information: 0 (need at least 5 columns per peak)
Peaks with misformatted coordinates: 0 (should be integer)
Peaks with misformatted strand: 0 (should be either +/- or 0/1)
Only 0 peaks found! If many more expected, maybe the file format is for Macs only
Try running the command: changeNewLine.pl "././target.clean..pos"
Background fragment size set to 0 (avg size of targets)
ls: ../../../Homer//mm9r.*.cgbins: No such file or directory
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