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Selecting of most differentially expressed transcripts

Hello all,

I did Differential Expression Analysis (using edgeR in Blast2GO Pro) for two groups at four different treatment time points, now I have to select most 25 differentially expressed transcripts (up and down regulated) for each time point for both groups and then I have to compare them but I don't know how to do that. I have to select them based on fold change.

Thanks in advance :)

rna-seq
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written by Valentina • Valentina

Thank you very much Devon! Do you know is it any possible way to do that in Blast2GO because later I want to compare the most DE transcripts between two groups based on GO ID.

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written by Valentina • Valentina

Please use ADD COMMENT/ADD REPLY when responding to existing posts to keep threads logically organized.

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written by GenoMax GenoMax

I suspect that you'll need to prefilter the input into Blast2GO, but I can't say for sure (I don't normally have any need for Blast2GO).

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written by Devon Ryan Devon Ryan

1 answer

First filter by adjusted p-value and then sort by the absolute value of the fold change. You can even do that in eXcel if you really want...

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written by Devon Ryan Devon Ryan

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