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FASTQC problem - repetitive k-mer

Hi, I ran fastqc on an RNA seq experiment, and the control's k-mer content is weird: around the middle of the read there is a strange k-mer peak where a specific k-mer is found. I've never seen anything like it before, especially as this is the control sample. could it be a matter of duplicates? Do you think removing duplicates would get rid of that?

Thanks!

rna-seq fastqc kmer
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written by blur 30

Might be helpful to show us the graph so we can judge how odd it is exactly. You could use http://tinypic.com or one of many alternatives for that.

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written by WouterDeCoster 4.8K

I'm behind a crazy firewall that blocks everything you can imagine... CGGTCTA count 4500 Obs/Exp Max - 19 position 24

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written by blur 30

Hi Could you post the image of the graph or describe it a bit more? How high is the Log2 Obs/Exp (table below graph).

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written by Jake Warner 84

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